Anales de la RANM

19 A N A L E S R A N M R E V I S T A F U N D A D A E N 1 8 7 9 S U P L E M E N T O I SIMPOSIO · JÓVENES INVESTIGADORES Libro de Abstracts An RANM. 2021;138(03).supl01: 19 - 54 On the other hand, we have analyzed other neuronal types in the hypothalamus such as GAD67-positive inhibitory neurons and dopaminergic neurons identified by the monoamine tyrosine hydroxy- lase (TH) marker. Our results indicate the presence of GAD67-positive neurons in the hypothalamus, but minimal co-localization in OXT or AVP cells. Furthermore, we found that sexual experience induces TH expression in a subpopulation of AVP and OXT neurons in the RCH. Our findings provide new fundamental information for understanding the specification of OXT and AVP systems during development and their plastic properties in response to critical periods in the adult animal. Figura 1. Formación de los circuitos de oxytocina (OXT) y vasopresina (AVP) durante el desarrollo del cerebro de ratón. A) La técnica de clarificación de tejido iDISCO+ permite obtener cerebros transparentes para su posterior tinción con anticuerpos y visualización mediante microscopía de ultra resolución Light Sheet y la posterior reconstrucción en 3D con una alta resolución celular mediante el programa Imaris. B) Mapas tridimensionales de los circuitos de OXT y AVP en la región hipotalámica durante el desarrollo. Los datos indican un número significativo de células de fenotipo mixto (OXT+AVP) a PN7. Barra de escala indica 200 µm. Figura modificada de Madrigal & Jurado, 2021. Figure 1. Formation of the oxytocin (OXT) and vasopressin (AVP) circuits during mouse brain development. A) The tissue clarifica- tion technique iDISCO+ allows to obtain transparent brains which can be further processed by immunohistochemistry and visua- lized with Light Sheet ultraresolution microscopy to 3D reconstruct the OXT and AVP circuits with the software Imaris. B) Three- dimensional maps of OXT and AVP circuits in the hypothalamus during neurodevelopment. Data indicates a significant number of cells which coexpress OXT and AVP at PN7. Scale bar indicates 200 µm. Figure modified from Madrigal & Jurado, 2021.